Exploring novel herbicidin analogues by transcriptional regulator overexpression and MS/MS molecular networking.

ABSTRACT:

Background

Herbicidin F has an undecose tricyclic furano-pyrano-pyran structure with post-decorations. It was detected from Streptomyces mobaraensis US-43 fermentation broth as a trace component by HPLC-MS analysis. As herbicidins exhibit herbicidal, antibacterial, antifungal and antiparasitic activities, we are attracted to explore more analogues for further development.

Results

The genome of S. mobaraensis US-43 was sequenced and a herbicidin biosynthetic gene cluster (hcd) was localized. The cluster contains structural genes, one transporter and three potential transcription regulatory genes. Overexpression of the three regulators respectively showed that only hcdR2 overexpression significantly improved the production of herbicidin F, and obviously increased the transcrip

SUBMITTER: Shi Y 

PROVIDER: S-EPMC6794829 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature