Establishment and optimization of mRNA in situ hybridization system in turnip (Brassica rapa var. rapa).

ABSTRACT:

Background

In situ hybridization (ISH) is a general molecular biological technique used to determine the spatiotemporal expression of genes in many species. In the past few years, numerous ISH protocols have been established in many species. Turnip (Brassica rapa var. rapa) is an important crop in the world, especially in the Plateau area of China, and is a traditional Tibetan medicine. However, ISH protocol in turnip has not been established.

Results

We explored and established an optimal workflow for mRNA ISH system for turnip which has been evaluated using BrrCLV3 and BrrWUSa. The optimal methods include: (1) fixation method, (2) protease K pretreatment time, (3) probe length and concentration, (4) washing temperature. We also provide advice on

SUBMITTER: Li C 

PROVIDER: S-EPMC6794860 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature