Identification of a noncanonical RNA binding domain in the U2 snRNP protein SF3A1.

ABSTRACT: During splicing of pre-mRNA, 5' and 3' splice sites are brought within proximity by interactions between the pre-mRNA bound U1 and U2 snRNPs, followed by recruitment of the tri-snRNP for assembly of the mature spliceosome. Previously, we identified an interaction between the U2 snRNP-specific protein SF3A1 and the stem-loop 4 (SL4) of the U1 snRNA that occurs during the early steps of spliceosome assembly. Although harboring many annotated domains, SF3A1 lacks a canonical RNA binding domain. To identify the U1-SL4 binding region in SF3A1, we expressed amino- and carboxy-terminal deletion constructs using a HeLa cell-based cell free expression system. UV-crosslinking of the truncated proteins with ³²P-U1-SL4 RNA identified the carboxy-terminal ubiquitin-like (UBL) domain of SF3A1

SUBMITTER: Martelly W 

PROVIDER: S-EPMC6795144 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature