Concordance between gene expression in peripheral whole blood and colonic tissue in children with inflammatory bowel disease.

ABSTRACT:

Background

Presenting features of inflammatory bowel disease (IBD) are non-specific. We hypothesized that mRNA profiles could (1) identify genes and pathways involved in disease pathogenesis; (2) identify a molecular signature that differentiates IBD from other conditions; (3) provide insight into systemic and colon-specific dysregulation through study of the concordance of the gene expression.

Methods

Children (8-18 years) were prospectively recruited at the time of diagnostic colonoscopy for possible IBD. We used transcriptome-wide mRNA profiling to study gene expression in colon biopsies and paired whole blood samples. Using blood mRNA measurements, we fit a regression model for disease state prediction that was validated in an independent test set of adult subjects (GSE3

SUBMITTER: Palmer NP 

PROVIDER: S-EPMC6795427 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature