A photoactivatable crosslinking system reveals protein interactions in the Toxoplasma gondii inner membrane complex.

ABSTRACT: The Toxoplasma gondii inner membrane complex (IMC) is an important organelle involved in parasite motility and replication. The IMC resides beneath the parasite's plasma membrane and is composed of both membrane and cytoskeletal components. Although the protein composition of the IMC is becoming better understood, the protein-protein associations that enable proper functioning of the organelle remain largely unknown. Determining protein interactions in the IMC cytoskeletal network is particularly challenging, as disrupting the cytoskeleton requires conditions that disrupt protein complexes. To circumvent this problem, we demonstrate the application of a photoreactive unnatural amino acid (UAA) crosslinking system to capture protein interactions in the native intracellular environment. In a

SUBMITTER: Choi CP 

PROVIDER: S-EPMC6795473 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature