Nanosecond photochemically promoted click chemistry for enhanced neuropeptide visualization and rapid protein labeling.

ABSTRACT: Comprehensive protein identification and concomitant structural probing of proteins are of great biological significance. However, this is challenging to accomplish simultaneously in one confined space. Here, we develop a nanosecond photochemical reaction (nsPCR)-based click chemistry, capable of structural probing of proteins and enhancing their identifications through on-demand removal of surrounding matrices within nanoseconds. The nsPCR is initiated using a photoactive compound, 2-nitrobenzaldehyde (NBA), and is examined by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). Benefiting from the on-demand matrix-removal effect, this nsPCR strategy enables enhanced neuropeptide identification and visualization from complex tissue samples such as mouse brain tissue.

SUBMITTER: Li G 

PROVIDER: S-EPMC6795811 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature