Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination.

ABSTRACT: BACKGROUND:Malaria elimination requires diagnostic methods able to detect parasite levels well below what is currently possible with microscopy and rapid diagnostic tests. This is particularly true in surveillance of malaria at the population level that includes so-called "asymptomatic" individuals. METHODS:The development of the first ultrasensitive loop mediated amplification method capable of detecting malaria from both whole blood and dried blood spots (DBS) is described. The 18S rRNA and corresponding genes that remain stable on DBS for up to 5 months are targeted. RESULTS:In the case of Plasmodium falciparum, lower limits of detection of 25 parasite/mL and 50-100 parasite/mL from whole blood and DBS were obtained, respectively. A sensitivity of 97.0% (95% CI 82.5-99.8) and specificit

SUBMITTER: Mohon AN 

PROVIDER: S-EPMC6796404 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature