Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi.

ABSTRACT:

Background

Within the last years, numerous reports described successful application of the CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi. However, still a lot of efforts are invested to develop and improve protocols for the fungus and genes of interest with respect to applicability, scalability and targeting efficiencies. These efforts are often hampered by the fact that-although many different protocols are available-none have systematically analysed and compared different CRISPR nucleases and different application procedures thereof for the efficiency of single- and multiplex-targeting approaches in the same fungus.

Results

We present here data for successful genome editing in the cell factory Thermothelomyces thermophilus, formerly known as

SUBMITTER: Kwon MJ 

PROVIDER: S-EPMC6796461 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature