Rapid one-step enzyme immunoassay and lateral flow immunochromatographic assay for colistin in animal feed and food.

ABSTRACT:

Background

Colistin (polymyxin E) is a kind of peptide antibiotic which has been approved in animal production for the purposes of disease prevention, treatment, and growth promotion. However, the wide use of colistin in animal feed may accelerate the spread of colistin-resistance gene MCR-1 from animal production to human beings, and its residue in animal-origin food may also pose serious health hazards to humans. Thus, it is necessary to develop corresponding analytical methods to monitor the addition of colistin in animal feed and the colistin residue in animal-origin food.

Results

A one-step enzyme-linked immunosorbent assay (ELISA) and a lateral flow immunochromatographic assay (LFIA) for colistin were developed based on a newly developed monoclonal antibody. The ELISA

SUBMITTER: Wang J 

PROVIDER: S-EPMC6796504 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature