N⁶-methyladenosine modification of ITGA6 mRNA promotes the development and progression of bladder cancer.

ABSTRACT:

Background

Accumulating evidence has revealed the critical roles of N⁶-methyladenosine (m⁶A) modification of mRNA in various cancers. However, the biological function and regulation of m⁶A in bladder cancer (BC) are not yet fully understood.

Methods

We performed cell phenotype analysis and established in vivo mouse xenograft models to assess the effects of m⁶A-modified ITGA6 on BC growth and progression. Methylated RNA immunoprecipitation (MeRIP), RNA immunoprecipitation and luciferase reporter and mutagenesis assays were used to define the mechanism of m⁶A-modified ITGA6. Immunohistochemical analysis was performed to assess the correlation between METTL3 and ITGA6 expression in bladder cancer patients.

Findings

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SUBMITTER: Jin H 

PROVIDER: S-EPMC6796523 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature