B7-H3-redirected chimeric antigen receptor T cells target glioblastoma and neurospheres.

ABSTRACT:

Background

The dismal survival of glioblastoma (GBM) patients urgently calls for the development of new treatments. Chimeric antigen receptor T (CAR-T) cells are an attractive strategy, but preclinical and clinical studies in GBM have shown that heterogeneous expression of the antigens targeted so far causes tumor escape, highlighting the need for the identification of new targets. We explored if B7-H3 is a valuable target for CAR-T cells in GBM.

Methods

We compared mRNA expression of antigens in GBM using TCGA data, and validated B7-H3 expression by immunohistochemistry. We then tested the antitumor activity of B7-H3-redirected CAR-T cells against GBM cell lines and patient-derived GBM neurospheres in vitro and in xenograft murine models.

Findings

B7-H3 mRNA and prot

SUBMITTER: Nehama D 

PROVIDER: S-EPMC6796553 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature