Application of new host biomarker profiles in quantitative point-of-care tests facilitates leprosy diagnosis in the field.

ABSTRACT:

Background

Transmission of Mycobacterium leprae, the pathogen causing leprosy, is still persistent. To facilitate timely (prophylactic) treatment and reduce transmission it is vital to both early diagnose leprosy, and identify infected individuals lacking clinical symptoms. However, leprosy-specific biomarkers are limited, particularly for paucibacillary disease. Therefore, our objective was to identify new biomarkers for leprosy and assess their applicability in point-of-care (POC) tests.

Methods

Using multiplex-bead-arrays, 60 host-proteins were measured in a cross-sectional approach in 24-h whole blood assays (WBAs) collected in Bangladesh (79 patients; 54 contacts; 51 endemic controls (EC)). Next, 17 promising biomarkers were validated in WBAs of a separate cohort (55 pa

SUBMITTER: van Hooij A 

PROVIDER: S-EPMC6796558 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature