Blockade of the kallikrein-kinin system reduces endothelial complement activation in vascular inflammation.

ABSTRACT:

Background

The complement and kallikrein-kinin systems (KKS) are activated during vascular inflammation. The aim of this study was to investigate if blockade of the KKS can affect complement activation on the endothelium during inflammation.

Methods

Complement deposition on endothelial microvesicles was assayed in vasculitis patient plasma samples and controls. Plasma was perfused over glomerular endothelial cells and complement deposition assayed by flow cytometry. The effect of the kinin system was assessed using kinin receptor antagonists and C1-inhibitor. The in vivo effect was assessed in kidney sections from mice with nephrotoxic serum-induced glomerulonephritis treated with a kinin receptor antagonist.

Findings

Vasculitis patient plasma had significantly more C

SUBMITTER: Lopatko Fagerstrom I 

PROVIDER: S-EPMC6796560 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature