Studying Interactions between 2'-O-Me-Modified Inhibitors and MicroRNAs Utilizing Microscale Thermophoresis.

ABSTRACT: Besides the acquisition of pharmacokinetic parameters of antisense oligonucleotide microRNA (miRNA) inhibitors, such as measuring in vivo concentration, their pharmacodynamic characteristics are also of interest. An emerging and straightforward method for studying molecular interactions is microscale thermophoresis (MST). This technique makes it possible to study interactions between miRNAs and various oligonucleotide inhibitors, independent of the chemical modifications of the inhibitors or their respective target structure, with very little sample volume required compared to competitive techniques, such as surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC). Interaction studies between these inhibitors and their respective target structures were performed, and they

SUBMITTER: Herkt M 

PROVIDER: S-EPMC6796726 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature