Analysis of a fully infectious bio-orthogonally modified human virus reveals novel features of virus cell entry.

ABSTRACT: We report the analysis of a complex enveloped human virus, herpes simplex virus (HSV), assembled after in vivo incorporation of bio-orthogonal methionine analogues homopropargylglycine (HPG) or azidohomoalanine (AHA). We optimised protocols for the production of virions incorporating AHA (termed HSVAHA), identifying conditions which resulted in normal yields of HSV and normal particle/pfu ratios. Moreover we show that essentially every single HSVAHA capsid-containing particle was detectable at the individual particle level by chemical ligation of azide-linked fluorochromes to AHA-containing structural proteins. This was a completely specific chemical ligation, with no capsids assembled under normal methionine-containing conditions detected in parallel. We demonstrate by quantitative mass s

SUBMITTER: Serwa RA 

PROVIDER: S-EPMC6797222 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature