ASPEN, a methodology for reconstructing protein evolution with improved accuracy using ensemble models.

ABSTRACT: Evolutionary reconstruction algorithms produce models of the evolutionary history of proteins or species. Such algorithms are highly sensitive to their inputs: the sequences used and their alignments. Here, we asked whether the variance introduced by selecting different input sequences could be used to better identify accurate evolutionary models. We subsampled from available ortholog sequences and measured the distribution of observed relationships between paralogs produced across hundreds of models inferred from the subsamples. We observed two important phenomena. First, the reproducibility of an all-sequence, single-alignment reconstruction, measured by comparing topologies inferred from 90% subsamples, directly correlates with the accuracy of that single-alignment reconstruction, produ

SUBMITTER: Sloutsky R 

PROVIDER: S-EPMC6797483 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature