MiR-640 aggravates intervertebral disc degeneration via NF-κB and WNT signalling pathway.

ABSTRACT:

Objectives

Low back pain becomes a common orthopaedic disease today. It is mainly induced by the degeneration of the intervertebral disc. In this study, we tried to reveal the pathogenesis of the degeneration and the relative therapeutic strategy, which are still elusive.

Materials and methods

We collected 15 degenerative intervertebral tissues and five healthy donors. Nucleus pulposus and annulus fibrosus cells were subcultured. miR-640 expression was determined by qPCR. Computer analysis and luciferase reporter assay were used to confirm miR-640 target genes. Immunohistochemical and immunocytochemical staining was used to trace the proinflammatory cytokines and key transductor of signalling pathways. We also used β-galactosidase staining, flow cytometry, and cell viability

SUBMITTER: Dong W 

PROVIDER: S-EPMC6797513 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature