Exosomes derived from miR-375-overexpressing human adipose mesenchymal stem cells promote bone regeneration.

ABSTRACT:

Objectives

The present study aimed to investigate whether exosomes derived from miR-375-overexpressing human adipose mesenchymal stem cells (hASCs) could enhance bone regeneration.

Materials and methods

Exosomes enriched with miR-375 (Exo [miR-375]) were generated from hASCs stably overexpressing miR-375 after lentiviral transfection and identified with transmission electron microscopy, nanosight and western blotting. The construction efficiency of Exo (miR-375) was evaluated with qRT-PCR and incubated with human bone marrow mesenchymal stem cells (hBMSCs) to optimize the effective dosage. Then, the osteogenic capability of Exo (miR-375) was investigated with ALP and ARS assays. Furthermore, dual-luciferase reporter assay and western blotting were conducted to reveal the und

SUBMITTER: Chen S 

PROVIDER: S-EPMC6797519 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature