Small GTPase RHOE/RND3, a new critical regulator of NF-κB signalling in glioblastoma multiforme?

ABSTRACT:

Objectives

Abnormal activation of NF-κB signalling is a major mechanism of apoptosis resistance in glioblastoma multiforme (GBM). Therefore, better understanding of the regulation of NF-κB signalling has a significant impact for GBM therapy. Here, we uncovered a critical role of the small GTPase RND3 in regulating the p65 subunit of NF-κB and NF-κB signalling in GBM.

Materials and methods

Human GBM samples, GBM cells and a human orthotopic GBM-xenografted animal model were used. The mechanisms of RND3 in regulation of NF-κB signalling and GBM cell apoptosis were examined by luciferase assay, quantitative PCR, immunostaining, immunoblotting, immunofluorescence, coimmunoprecipitation, TUNEL staining, JC-1 analysis and flow cytometry.

Results

Overexpression of RND3 led t

SUBMITTER: Sun Q 

PROVIDER: S-EPMC6797521 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature