14-3-3ζ targeting induced senescence in Hep-2 laryngeal cancer cell through deneddylation of Cullin1 in the Skp1-Cullin-F-box protein complex.

ABSTRACT:

Objectives

Despite of the aberrant expression of 14-3-3ζ in head and neck squamous cell carcinoma (HNSCC), little is known about the role of 14-3-3ζ in the regulation of senescence in HNSCC. This study was performed to investigate whether 14-3-3ζ is implicated in senescence evasion of Hep-2 laryngeal cancer cells.

Methods

The expression of 14-3-3ζ was suppressed using RNA interference strategy. Senescence induction was determined by senescence-associated β-galactosidase staining and the numbers of promyelocytic leukaemia nuclear body. Real-time PCR, western blotting and immunohistochemistry were applied for the expression of corresponding proteins. Xenograft experiment was performed to show in vivo effect of 14-3-3ζ silencing on tumour growth.

Results

14-3-3ζ silencin

SUBMITTER: Seo SB 

PROVIDER: S-EPMC6797561 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature