Differences in gene expression between high-grade dysplasia and invasive HPV⁺ and HPV^- tonsillar and base of tongue cancer.

ABSTRACT:

Background

Human papillomavirus (HPV) is a causative agent for tonsillar and base of tongue squamous cell carcinoma (TSCC/BOTSCC), as well as for cervical cancer. Premalignant stages in cervical cancer have been studied extensively, while little is known about premalignant stages in TSCC/BOTSCC and the role of HPV. Here we analyzed differences in gene and protein expression between high-grade dysplasia and invasive cancer in both HPV-positive (HPV⁺ ) and HPV-negative (HPV^- ) TSCC/BOTSCC.

Methods

High-grade dysplasia and invasive carcinoma were laser microdissected from HPV⁺ and HPV^- TSCC/BOTSCC tumor sections. Differential gene expression was studied utilizing nanoString RNA-panels and genes of interest were validated on the protei

SUBMITTER: Haeggblom L 

PROVIDER: S-EPMC6797576 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature