Chitosan enhances rosmarinic acid production in shoot cultures of Melissa officinalis L. through the induction of methyl jasmonate.

ABSTRACT: BACKGROUND:Chitosan is a polycationic polysaccharide derived from chitin that has been recognized as an effective elicitor in the production of secondary metabolites of many medicinal plants. In this study, the effect of abiotic elicitor (chitosan) at various concentrations on rosmarinic acid (RA) and total phenolic accumulation in shoot cultures of lemon balm was investigated. RESULTS:Treatment of shoots by chitosan led to a noticeable induction of phenylalanine ammonia-lyase (PAL), catalase (CAT), guaiacol peroxidase (GPX) and lipoxygenase (LOX) activities. Besides, the expression of PAL1, TAT and RAS genes and accumulation of RA and phenolic compound increased in chitosan-treated lemon balm shoots. Chitosan treatment also increased H2O2 accumulation and the expression of RBOH, an essent

SUBMITTER: Fooladi Vanda G 

PROVIDER: S-EPMC6797681 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature