Transcriptome profiling of mouse samples using nanopore sequencing of cDNA and RNA molecules.

ABSTRACT: Our vision of DNA transcription and splicing has changed dramatically with the introduction of short-read sequencing. These high-throughput sequencing technologies promised to unravel the complexity of any transcriptome. Generally gene expression levels are well-captured using these technologies, but there are still remaining caveats due to the limited read length and the fact that RNA molecules had to be reverse transcribed before sequencing. Oxford Nanopore Technologies has recently launched a portable sequencer which offers the possibility of sequencing long reads and most importantly RNA molecules. Here we generated a full mouse transcriptome from brain and liver using the Oxford Nanopore device. As a comparison, we sequenced RNA (RNA-Seq) and cDNA (cDNA-Seq) molecules using both long

SUBMITTER: Sessegolo C 

PROVIDER: S-EPMC6797730 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature