Role of CCR7 on dendritic cell‑mediated immune tolerance in the airways of allergy‑induced asthmatic rats.

ABSTRACT: Dendritic cells (DCs) have an important role in initiating and maintaining the immune inflammatory response in allergic asthma, and CC chemokine receptor 7 (CCR7) is directly involved in the pathogenesis of DC‑ and T cell‑mediated allergic asthma. The present study aimed to investigate the effects of CCR7 on DC‑mediated immune tolerance in allergic asthma. In the present study, bone marrow‑derived DCs were transfected with an adenovirus encoding the rat CCR7 gene or a short hairpin RNA targeting CCR7 (sh‑CCR7). Rats injected with DCs overexpressing CCR7 or presenting CCR7 knockdown were examined. After the rats were injected with DCs via the tail vein, bronchoalveolar lavage fluid was collected to assess its cellular composition. The protein expression levels of CCR7 in DCs were determined

SUBMITTER: Li Y 

PROVIDER: S-EPMC6797982 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature