A Robust and General Approach to Quantitatively Conjugate Enzymes to Plasmonic Nanoparticles.

ABSTRACT: Bioconjugates of plasmonic nanoparticles have received considerable attention due to their potential biomedical applications. Successful bioconjugation requires control over the number and activity of the conjugated proteins and the colloidal stability of the particles. In practice, this requires reoptimization of the conjugation protocol for each combination of protein and nanoparticle. Here, we report a robust and general protocol that allows for the conjugation of a range of proteins to different types of nanoparticles using very short polyethylene-glycol(PEG) linkers, while simultaneously preserving protein activity and colloidal stability. The use of short linkers ensures that the protein is located close to the particle surface, where the refractive index sensitivity and near-field e

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6798157 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature