Phenotypic Characterization of Chinese Rhesus Macaque Plasmablasts for Cloning Antigen-Specific Monoclonal Antibodies.

ABSTRACT: Rhesus macaques (Macaca mulatta) are used as a human-relevant animal species for the evaluation of vaccines and as a source for cloning monoclonal antibodies (mAbs) that are highly similar to human-derived antibodies. Although antibody-secreting plasmablasts in humans are well-defined and can be easily isolated for mAb cloning, it remains unclear whether the same phenotypic markers could be applied for isolating antibody-secreting plasmablasts from Chinese rhesus macaques. In this study, we evaluated a series of cell surface and intracellular markers and identified the phenotypic markers of plasmablasts in Chinese rhesus macaques as CD3^-CD14^-CD56^-CD19^-CD27^-CD20^-/lowCD80⁺HLA-DR⁺CD95⁺. A

SUBMITTER: Zhang F 

PROVIDER: S-EPMC6798180 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature