Performance characteristics of a modified HIV-1 drug resistance genotyping method for use in resource-limited settings.

ABSTRACT: Background: HIV-1 drug resistance (HIVDR) assays are critical components of HIV clinical management programs in the face of emerging drug resistance. However, the high costs associated with existing commercial HIVDR assays prohibit their routine usage in resource-limited settings. We present the performance characteristics of a modified commercial HIVDR testing assay. Methods: A total of 26 plasma samples were used to validate and assess the accuracy, precision, reproducibility and amplification sensitivity of a modified HIVDR assay by HIV genotyping. In addition, a cost comparison between the original and the modified assay was performed using the ingredient costing approach. Results: The performance characteristics of the modified assay were in agreement with the original assay. Accuracy

SUBMITTER: Magomere EO 

PROVIDER: S-EPMC6798314 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature