PH-triggered endosomal escape of pore-forming Listeriolysin O toxin-coated gold nanoparticles.

ABSTRACT:

Background

A major bottleneck in drug delivery is the breakdown and degradation of the delivery system through the endosomal/lysosomal network of the host cell, hampering the correct delivery of the drug of interest. In nature, the bacterial pathogen Listeria monocytogenes has developed a strategy to secrete Listeriolysin O (LLO) toxin as a tool to escape the eukaryotic lysosomal system upon infection, allowing it to grow and proliferate unharmed inside the host cell.

Results

As a "proof of concept", we present here the use of purified His-LLO H311A mutant protein and its conjugation on the surface of gold nanoparticles to promote the lysosomal escape of 40 nm-sized nanoparticles in mouse embryonic fibroblasts. Surface immobilization of LLO was achieved after specific functi

SUBMITTER: Plaza-Ga I 

PROVIDER: S-EPMC6798460 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature