A paper-based microfluidic platform with shape-memory-polymer-actuated fluid valves for automated multi-step immunoassays.

ABSTRACT: Smart fluid manipulation with automatically controlled paper valves will enable automated and multi-step immunoassays on paper-based microfluidic devices. In this work, we present an integrated paper-based microfluidic platform with shape-memory polymer (SMP)-actuated fluid valves capable of automated colorimetric enzyme-linked immunosorbent assays (ELISAs). A single-layer microfluidic paper-based analytical device (μPAD) was designed to store all the reagents on the chip, and sequentially transfer reagents to a paper test zone following a specific ELISA protocol through automatic fluidic flow control by the multiple SMP-actuated valves. The actuation of a paper valve was based on the thermally responsive, duel-state shape transformation of a SMP sheet attached to the root of a paper canti

SUBMITTER: Fu H 

PROVIDER: S-EPMC6799814 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature