The Ca²⁺ export pump PMCA clears near-membrane Ca²⁺ to facilitate store-operated Ca²⁺ entry and NFAT activation.

ABSTRACT: Ca²⁺ signals, which facilitate pluripotent changes in cell fate, reflect the balance between cation entry and export. We found that overexpression of either isoform of the Ca²⁺-extruding plasma membrane calcium ATPase 4 (PMCA4) pump in Jurkat T cells unexpectedly increased activation of the Ca²⁺-dependent transcription factor nuclear factor of activated T cells (NFAT). Coexpression of the endoplasmic reticulum-resident Ca²⁺ sensor stromal interaction molecule 1 (STIM1) with the PMCA4b splice variant further enhanced NFAT activity; however, coexpression with PMCA4a depressed NFAT. No PMCA4 splice variant dependence in STIM1 association was observed, whereas partner of STIM1 (POST) preferentially associated with PMCA4b over PMCA4a, which enhanced,

SUBMITTER: Go CK 

PROVIDER: S-EPMC6800072 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature