Species Typing of Nontuberculous Mycobacteria by Use of Deoxyribozyme Sensors.

ABSTRACT:

Background

Nontuberculous mycobacteria (NTM) species are a rising threat, especially to patients living with pulmonary comorbidities. Current point-of-care diagnostics fail to adequately identify and differentiate NTM species from Mycobacterium tuberculosis (Mtb). Definitive culture- and molecular-based testing can take weeks to months and requires sending samples out to specialized diagnostic laboratories.

Methods

In this proof-of-concept study, we developed an assay based on PCR amplification of 16S ribosomal RNA (rRNA) rrs genes by using universal mycobacterial primers and interrogation of the amplified fragments with a panel of binary deoxyribozyme (BiDz) sensors to enable species-level identification of NTM (BiDz-NTM_ST). Each BiDz sensor

SUBMITTER: Wood HN 

PROVIDER: S-EPMC6800115 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature