Genomic Decoding of Neuronal Depolarization by Stimulus-Specific NPAS4 Heterodimers.

ABSTRACT: Cells regulate gene expression in response to salient external stimuli. In neurons, depolarization leads to the expression of inducible transcription factors (ITFs) that direct subsequent gene regulation. Depolarization encodes both a neuron's action potential (AP) output and synaptic inputs, via excitatory postsynaptic potentials (EPSPs). However, it is unclear if distinct types of electrical activity can be transformed by an ITF into distinct modes of genomic regulation. Here, we show that APs and EPSPs in mouse hippocampal neurons trigger two spatially segregated and molecularly distinct induction mechanisms that lead to the expression of the ITF NPAS4. These two pathways culminate in the formation of stimulus-specific NPAS4 heterodimers that exhibit distinct DNA binding patterns. Thus,

SUBMITTER: Brigidi GS 

PROVIDER: S-EPMC6800120 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature