Nosocomial transmission of influenza: A retrospective cross-sectional study using next generation sequencing at a hospital in England (2012-2014).

ABSTRACT:

Background

The extent of transmission of influenza in hospital settings is poorly understood. Next generation sequencing may improve this by providing information on the genetic relatedness of viral strains.

Objectives

We aimed to apply next generation sequencing to describe transmission in hospital and compare with methods based on routinely-collected data.

Methods

All influenza samples taken through routine care from patients at University College London Hospitals NHS Foundation Trust (September 2012 to March 2014) were included. We conducted Illumina sequencing and identified genetic clusters. We compared nosocomial transmission estimates defined using classical methods (based on time from admission to sample) and genetic clustering. We identified pairs of cases wi

SUBMITTER: Blackburn RM 

PROVIDER: S-EPMC6800305 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature