FeedER: a feedback-regulated enzyme-based slow-release system for fed-batch cultivation in microtiter plates.

ABSTRACT: With the advent of modern genetic engineering methods, microcultivation systems have become increasingly important tools for accelerated strain phenotyping and bioprocess engineering. While these systems offer sophisticated capabilities to screen batch processes, they lack the ability to realize fed-batch processes, which are used more frequently in industrial bioprocessing. In this study, a novel approach to realize a feedback-regulated enzyme-based slow-release system (FeedER), allowing exponential fed-batch for microscale cultivations, was realized by extending our existing Mini Pilot Plant technology with a customized process control system. By continuously comparing the experimental growth rates with predefined set points, the automated dosage of Amyloglucosidase enzyme for the cleava

SUBMITTER: Jansen R 

PROVIDER: S-EPMC6800402 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature