Distinct substrate specificities of the human tRNA methyltransferases TRMT10A and TRMT10B.

ABSTRACT: The tRNA m¹R₉ methyltransferase (Trm10) family is conserved throughout Eukarya and Archaea. Despite the presence of a single Trm10 gene in Archaea and most single-celled eukaryotes, metazoans encode up to three homologs of Trm10. Several disease states correlate with a deficiency in the human homolog TRMT10A, despite the presence of another cytoplasmic enzyme, TRMT10B. Here we investigate these phenomena and demonstrate that human TRMT10A (hTRMT10A) and human TRMT10B (hTRMT10B) are not biochemically redundant. In vitro activity assays with purified hTRMT10A and hTRMT10B reveal a robust activity for hTRMT10B as a tRNA^Asp-specific m¹A₉ methyltransferase and suggest that it is the relevant enzyme responsible for this newly discovered m

SUBMITTER: Howell NW 

PROVIDER: S-EPMC6800469 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature