Determinants of target prioritization and regulatory hierarchy for the bacterial small RNA SgrS.

ABSTRACT: Small RNA (sRNA) regulators promote efficient responses to stress, but the mechanisms for prioritizing target mRNA regulation remain poorly understood. This study examines mechanisms underlying hierarchical regulation by the sRNA SgrS, found in enteric bacteria and produced under conditions of metabolic stress. SgrS posttranscriptionally coordinates a nine-gene regulon to restore growth and homeostasis. An in vivo reporter system quantified SgrS-dependent regulation of target genes and established that SgrS exhibits a clear target preference. Regulation of some targets is efficient even at low SgrS levels, whereas higher SgrS concentrations are required to regulate other targets. In vivo and in vitro analyses revealed that RNA structure and the number and position of base pairing sites rel

SUBMITTER: Bobrovskyy M 

PROVIDER: S-EPMC6800775 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature