Quantification and Localization of Protein-RNA Interactions in Patient-Derived Archival Tumor Tissue.

ABSTRACT: Abnormal post-transcriptional regulation induced by alterations of mRNA-protein interactions is critical during tumorigenesis and cancer progression and is a hallmark of cancer cells. A more thorough understanding is needed to develop treatments and foresee outcomes. Cellular and mouse tumor models are insufficient for vigorous investigation as they lack consistency and translatability to humans. Moreover, to date, studies in human tumor tissue are predominately limited to expression analysis of proteins and mRNA, which do not necessarily provide information about the frequency of mRNA-protein interactions. Here, we demonstrate novel optimization of a method that is based on FISH and proximity ligation techniques to quantify mRNA interactions with RNA-binding proteins relevant for tumorige

SUBMITTER: Blanchard EL 

PROVIDER: S-EPMC6801105 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature