One-vector CRISPR/Cas9 genome engineering of the industrial fungus Ashbya gossypii.

ABSTRACT: The filamentous fungus Ashbya gossypii is currently used for the industrial production of vitamin B2. Furthermore, the ability of A. gossypii to grow using low-cost substrates together with the inexpensive downstream processing makes this fungus an attractive biotechnological chassis. Indeed, the production in A. gossypii of other high-added value compounds such as folic acid, nucleosides and biolipids has been described. Hence, the development of new methods to expand the molecular toolkit for A. gossypii genomic manipulation constitutes an important issue for the biotechnology of this fungus. In this work, we present a one-vector CRISPR/Cas9 system for genomic engineering of A. gossypii. We demonstrate the efficiency of the system as a marker-less approach for nucleotide deletions and su

SUBMITTER: Jimenez A 

PROVIDER: S-EPMC6801137 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature