GRP 78 antibodies are associated with clinical phenotype in neuromyelitis optica.

ABSTRACT:

Background

We previously reported the association between blood-brain barrier (BBB) dysfunction and glucose-regulated protein 78 (GRP 78) autoantibodies in neuromyelitis optica (NMO).

Objective

We clarify whether the BBB-endothelial cell activation induced by immunoglobulin G (IgG) is associated with the clinical phenotype, disease activity, and markers of BBB disruption.

Methods

We purified serum IgG from 24 serum samples from patients with NMO spectrum disorder (NMOSD), who were positive for anti-AQP4 antibodies (longitudinally extensive transverse myelitis [LETM], n = 14; optic neuritis [ON], n = 6; other phenotype, n = 4) and nine healthy controls. IgG was exposed to human brain microvascular endothelial cells (TY10) and the number of nuclear NF-κB p65-positive ce

SUBMITTER: Shimizu F 

PROVIDER: S-EPMC6801168 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature