Cultivation technology development of Rhodothermus marinus DSM 16675.

ABSTRACT: This work presents an evaluation of batch, fed-batch, and sequential batch cultivation techniques for production of R. marinus DSM 16675 and its exopolysaccharides (EPSs) and carotenoids in a bioreactor, using lysogeny broth (LB) and marine broth (MB), respectively, in both cases supplemented with 10 g/L maltose. Batch cultivation using LB supplemented with maltose (LB_malt) resulted in higher cell density (OD₆₂₀ = 6.6) than use of MB_malt (OD₆₂₀ = 1.7). Sequential batch cultivation increased the cell density threefold (OD₆₂₀ = 20) in LB_malt and eightfold (OD₆₂₀ = 14) in MB_malt. In both single and sequential batches, the production of carotenoids and EPSs using LB_malt was detected in the expone

SUBMITTER: Ron EYC 

PROVIDER: S-EPMC6801211 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature