Causes and Consequences of A Glutamine Induced Normoxic HIF1 Activity for the Tumor Metabolism.

ABSTRACT: The transcription factor hypoxia-inducible factor 1 (HIF1) is the crucial regulator of genes that are involved in metabolism under hypoxic conditions, but information regarding the transcriptional activity of HIF1 in normoxic metabolism is limited. Different tumor cells were treated under normoxic and hypoxic conditions with various drugs that affect cellular metabolism. HIF1α was silenced by siRNA in normoxic/hypoxic tumor cells, before RNA sequencing and bioinformatics analyses were performed while using the breast cancer cell line MDA-MB-231 as a model. Differentially expressed genes were further analyzed and validated by qPCR, while the activity of the metabolites was determined by enzyme assays. Under normoxic conditions, HIF1 activity was significantly increased by (i) glutamine meta

SUBMITTER: Kappler M 

PROVIDER: S-EPMC6802203 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature