Hematopoietic Npc1 mutation shifts gut microbiota composition in Ldlr^-/- mice on a high-fat, high-cholesterol diet.

ABSTRACT: While the link between diet-induced changes in gut microbiota and lipid metabolism in metabolic syndrome (MetS) has been established, the contribution of host genetics is rather unexplored. As several findings suggested a role for the lysosomal lipid transporter Niemann-Pick type C1 (NPC1) in macrophages during MetS, we here explored whether a hematopoietic Npc1 mutation, induced via bone marrow transplantation, influences gut microbiota composition in low-density lipoprotein receptor knockout (Ldlr^-/-) mice fed a high-fat, high-cholesterol (HFC) diet for 12 weeks. Ldlr^-/- mice fed a HFC diet mimic a human plasma lipoprotein profile and show features of MetS, providing a model to explore the role of host genetics on gut microbiota under MetS conditions. Fecal samples

SUBMITTER: Houben T 

PROVIDER: S-EPMC6802207 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature