A magnetics-based approach for fine-tuning afterload in engineered heart tissues.

ABSTRACT: Afterload plays important roles during heart development and disease progression, however, studying these effects in a laboratory setting is challenging. Current techniques lack the ability to precisely and reversibly alter afterload over time. Here, we describe a magnetics-based approach for achieving this control and present results from experiments in which this device was employed to sequentially increase afterload applied to rat engineered heart tissues (rEHTs) over a 7-day period. The contractile properties of rEHTs grown on control posts marginally increased over the observation period. The average post deflection, fractional shortening, and twitch velocities measured for afterload-affected tissues initially followed this same trend, but fell below control tissue values at high magn

SUBMITTER: Rodriguez ML 

PROVIDER: S-EPMC6803165 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature