Discovery and Characterization of Native Deinococcus radiodurans Promoters for Tunable Gene Expression.

ABSTRACT: The potential utilization of extremophiles as a robust chassis for metabolic engineering applications has prompted interest in the use of Deinococcus radiodurans for bioremediation efforts, but current applications are limited by the lack of availability of genetic tools, such as promoters. In this study, we used a combined computational and experimental approach to identify and screen 30 predicted promoters for expression in D. radiodurans using a fluorescent reporter assay. The top eight candidates were further characterized, compared to currently available promoters, and optimized for engineering through minimization for use in D. radiodurans Of these top eight, two promoter regions, PDR_1261 and PrpmB, were stronger and more consistent than the most w

SUBMITTER: Chen A 

PROVIDER: S-EPMC6803307 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature