Genetic heterogeneity within collective invasion packs drives leader and follower cell phenotypes.

ABSTRACT: Collective invasion, the coordinated movement of cohesive packs of cells, has become recognized as a major mode of metastasis for solid tumors. These packs are phenotypically heterogeneous and include specialized cells that lead the invasive pack and others that follow behind. To better understand how these unique cell types cooperate to facilitate collective invasion, we analyzed transcriptomic sequence variation between leader and follower populations isolated from the H1299 non-small cell lung cancer cell line using an image-guided selection technique. We now identify 14 expressed mutations that are selectively enriched in leader or follower cells, suggesting a novel link between genomic and phenotypic heterogeneity within a collectively invading tumor cell population. Functional charac

SUBMITTER: Zoeller EL 

PROVIDER: S-EPMC6803364 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature