H3K27me3-mediated silencing of structural genes is required for zebrafish heart regeneration.

ABSTRACT: Deciphering the genetic and epigenetic regulation of cardiomyocyte proliferation in organisms that are capable of robust cardiac renewal, such as zebrafish, represents an attractive inroad towards regenerating the human heart. Using integrated high-throughput transcriptional and chromatin analyses, we have identified a strong association between H3K27me3 deposition and reduced sarcomere and cytoskeletal gene expression in proliferative cardiomyocytes following cardiac injury in zebrafish. To move beyond an association, we generated an inducible transgenic strain expressing a mutant version of histone 3, H3.3^K27M, that inhibits H3K27me3 catalysis in cardiomyocytes during the regenerative window. Hearts comprising H3.3^K27M-expressing cardiomyocytes fail to regenerate, w

SUBMITTER: Ben-Yair R 

PROVIDER: S-EPMC6803378 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature