Predicting and Experimentally Validating Hot-Spot Residues at Protein-Protein Interfaces.

ABSTRACT: Protein-protein interactions (PPIs) are vital to all biological processes. These interactions are often dynamic, sometimes transient, typically occur over large topographically shallow protein surfaces, and can exhibit a broad range of affinities. Considerable progress has been made in determining PPI structures. However, given the above properties, understanding the key determinants of their thermodynamic stability remains a challenge in chemical biology. An improved ability to identify and engineer PPIs would advance understanding of biological mechanisms and mutant phenotypes and also provide a firmer foundation for inhibitor design. In silico prediction of PPI hot-spot amino acids using computational alanine scanning (CAS) offers a rapid approach for predicting key residues that

SUBMITTER: Ibarra AA 

PROVIDER: S-EPMC6804253 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature