Impaired development of a miltefosine-resistant Leishmania infantum strain in the sand fly vectors Phlebotomus perniciosus and Lutzomyia longipalpis.

ABSTRACT:

Objectives

To gain insight into the propagation of miltefosine (MIL) resistance in visceral leishmaniasis, this laboratory study explored development of resistant parasites with a defective miltefosine transporter (MT) in sand flies.

Methods

Infectivity, colonization of stomodeal valve and metacyclogenesis of a MIL-resistant (MIL-R) Leishmania infantum LEM3323 line with a defective MT were assessed in the natural sand fly vectors Phlebotomus perniciosus and Lutzomyia longipalpis. Given our recent description of partial drug dependency of the MT-deficient line, the impact of MIL pre-exposure on sand fly infectivity was explored as well.

Results

A significant reduction in sand fly infection, stomodeal valve colonization and differentiation into metacyclics (determined b

SUBMITTER: Van Bockstal L 

PROVIDER: S-EPMC6804374 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature