Comparison of pre-analytical conditions for quantification of serotonin in platelet-poor plasma.

ABSTRACT:

Background

Reported concentrations of serotonin in platelet-poor plasma (PPP) in healthy subjects vary widely due to different pre-analytical procedures.

Aim

To examine how different pre-analytical conditions affect the measured concentration of serotonin in PPP.

Method

Six pre-analytical protocols were compared for preparation of PPP from EDTA whole blood for quantification of serotonin from nine healthy individuals. Three combinations of centrifugation with a mild centrifugation of gel-free EDTA tubes followed by a stronger centrifugation were compared to single-stage centrifugation of EDTA tubes with separator gel and heat shock treatment of blood prior to centrifugation. All samples were analysed using the same enzyme linked immunosorbent assay (ELISA) method.

SUBMITTER: Von Volkmann HL 

PROVIDER: S-EPMC6804510 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature