TOR inhibition interrupts the metabolic homeostasis by shifting the carbon-nitrogen balance in Chlamydomonas reinhardtii.

ABSTRACT: The allocation of nutrient resources to growth and metabolism is an essential function for controlling biomass accumulation in photoautotrophic organisms. One essential protein complex involved in this process is the target of rapamycin (TOR) kinase. It has been shown that the inhibition of TOR leads to a considerable upsurge in the amino acid levels. This molecular phenotype relies mainly on the availability of light, carbon (C) and nitrogen (N). To validate the time-resolved response of C and N metabolites, we used a targeted gas chromatography mass spectrometery (GC-MS)-based metabolomic approach, where we examined the response of Chlamydomonas reinhardtii upon TOR inhibition under C-limited condition, namely extended darkness. Contrary to C-supplemented conditions, the rapid inc

SUBMITTER: Mubeen U 

PROVIDER: S-EPMC6804693 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature